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Probate is the judicial process whereby a will is 'proved' in a court of law and accepted as a valid public document that is the true last testament of the deceased, or whereby the estate is settled according to the laws of intestacy in the state of residence of the deceased at time of death in the absence of a legal will.

Category: Minimum Requirements: Recommended Requirements OS: WINDOWS 7 (32 bit) WINDOWS 7 (64 bit) CPU: Intel/AMD 2 (Ghz) Intel Core i3 or better: Memory. The executor must attach a statement to Form 1041 providing the following information for each electing trust (including the electing trust provided in Item G): (a) the name of the electing trust, (b) the TIN of the electing trust, and (c) the name and address of the trustee of the electing trust.

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Configuration file¶

When a pipeline script is launched Nextflow looks for a file named nextflow.config in the current directory andin the script base directory (if it is not the same as the current directory). Finally it checks for the file$HOME/.nextflow/config.

When more than one of the above files exist they are merged, so that the settings in the first override the same onesthat may appear in the second one, and so on.

Code

The default config file search mechanism can be extended proving an extra configuration file by using the command lineoption -c<configfile>.

Note

It’s worth noting that by doing this, the files nextflow.config and $HOME/.nextflow/config are notignored and they are merged as explained above.

Tip

If you want to ignore any default configuration files and use only the custom one use the command line option-C<configfile>.

Config syntax¶

A Nextflow configuration file is a simple text file containing a set of properties defined using the syntax:

Please note, string values need to be wrapped in quotation characters while numbers and boolean values (true, false) do not.Also note that values are typed, meaning for example that, 1 is different from '1', since the first is interpretedas the number one, while the latter is interpreted as a string value.

Config Variables¶

Configuration properties can be used as variables in the configuration file itself, by using the usual$propertyName or ${expression} syntax.

For example:

Please note, the usual rules for String interpolation are applied, thus a string containing a variablereference must be wrapped in double-quote chars instead of single-quote chars.

The same mechanism allows you to access environment variables defined in the hosting system. Any variable whose name isnot defined in the Nextflow configuration file(s) is supposed to be a reference to an environment variable with that name.So, in the above example the property customPath is defined as the current system PATH to whichthe string /my/app/folder is appended.

Config comments¶

Configuration files use the same conventions for comments used by the Groovy or Java programming languages. Thus, use // to commenta single line or /* .. */ to comment a block on multiple lines.

Config include¶

A configuration file can include one or more configuration files using the keyword includeConfig. For example:

When a relative path is used, it is resolved against the actual location of the including file.

Config scopes¶

Configuration settings can be organized in different scopes by dot prefixing the property names with a scopeidentifier or grouping the properties in the same scope using the curly brackets notation. This is shown in thefollowing example:

Scope env

The env scope allows the definition one or more variable that will be exported in the environment where theworkflow tasks will be executed.

Simply prefix your variable names with the env scope or surround them by curly brackets, as shown below:

Scope params

The params scope allows you to define parameters that will be accessible in the pipeline script. Simply prefix theparameter names with the params scope or surround them by curly brackets, as shown below:

Scope process

The process configuration scope allows you to provide the default configuration for the processes in your pipeline.

You can specify here any property described in the process directive and the executor sections.For examples:

By using this configuration all processes in your pipeline will be executed through the SGE cluster, with the specifiedsettings.

Process selectors¶

The withLabel selectors allow the configuration of all processes annotated with a label directive asshown below:

The above configuration example assigns 16 cpus, 64 Gb of memory and the long queue to all processes annotatedwith the big_mem label.

In the same manner, the withName selector allows the configuration of a specific process in your pipeline by its name.For example:

Tip

Either label and process names do not need to be enclosed with quote characters, provided the namedoes include special characters (e.g. -, !, etc) or it’s not a keyword or a built-in type identifier.In case of doubt, you can enclose the label names or the process names with single or double quote characters.

Selector expressions¶

Both label and process name selectors allow the use of a regular expression in order to apply the same configurationto all processes matching the specified pattern condition. For example:

The above configuration snippet sets 2 cpus and 4 GB of memory to the processes annotated with with a label fooand bar.

A process selector can be negated prefixing it with the special character !. For example:

The above configuration snippet sets 2 cpus for the processes annotated with the foo label and 4 cpus to all processesnot annotated with that label. Finally it sets the use of long queue to all process whose name does not startwith align.

Selectors priority¶

When mixing generic process configuration and selectors the following priority rules are applied (from lower to higher):

  1. Process generic configuration.

  2. Process specific directive defined in the workflow script.

  3. withLabel selector definition.

  4. withName selector definition.

For example:

Using the above configuration snippet, all workflow processes use 4 cpus if not otherwise specified in the workflowscript. Moreover processes annotated with the foo label use 8 cpus. Finally the process named baruses 32 cpus.

Scope executor

The executor configuration scope allows you to set the optional executor settings, listed in the following table.

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Name

Description

name

The name of the executor to be used e.g. local, sge, etc.

queueSize

The number of tasks the executor will handle in a parallel manner (default: 100).

pollInterval

Determines how often a poll occurs to check for a process termination.

dumpInterval

Determines how often the executor status is written in the application log file (default: 5min).

queueStatInterval

Determines how often the queue status is fetched from the cluster system. This setting is used only by grid executors (default: 1min).

exitReadTimeout

Determines how long the executor waits before return an error status when a process is terminated but the exit file does not exist or it is empty. This setting is used only by grid executors (default: 270sec).

killBatchSize

Determines the number of jobs that can be killed in a single command execution (default: 100).

submitRateLimit

Determines the max rate of jobs that can be executed per time unit, for example '10sec' eg. max 10 jobs per second (default: unlimited).

perJobMemLimit

Specifies Platform LSF per-job memory limit mode. See LSF.

jobName

Determines the name of jobs submitted to the underlying cluster executor e.g. executor.jobName={'$task.name-$task.hash'} .

cpus

The maximum number of CPUs made available by the underlying system (only used by the local executor).

memory

The maximum amount of memory made available by the underlying system (only used by the local executor).

The executor settings can be defined as shown below:

When using two (or more) different executors in your pipeline, you can specify their settings separately by prefixingthe executor name with the symbol $ and using it as special scope identifier. For example:

The above configuration example can be rewritten using the dot notation as shown below:

Scope docker

The docker configuration scope controls how Docker containers are executed by Nextflow.

The following settings are available:

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Name

Description

enabled

Turn this flag to true to enable Docker execution (default: false).

envWhitelist

Comma separated list of environment variable names to be included in the container environment.

legacy

Uses command line options removed since version 1.10.x (default: false).

sudo

Executes Docker run command as sudo (default: false).

tty

Allocates a pseudo-tty (default: false).

temp

Mounts a path of your choice as the /tmp directory in the container. Use the special value auto to create a temporary directory each time a container is created.

remove

Clean-up the container after the execution (default: true). For details see: https://docs.docker.com/engine/reference/run/#clean-up—rm .

runOptions

This attribute can be used to provide any extra command line options supported by the dockerrun command. For details see: https://docs.docker.com/engine/reference/run/ .

registry

The registry from where Docker images are pulled. It should be only used to specify a private registry server. It should NOT include the protocol prefix i.e. http://.

fixOwnership

Fixes ownership of files created by the docker container.

engineOptions

This attribute can be used to provide any option supported by the Docker engine i.e. docker[OPTIONS].

mountFlags

Add the specified flags to the volume mounts e.g. mountFlags = ‘ro,Z’

The above options can be used by prefixing them with the docker scope or surrounding them by curlybrackets, as shown below:

Read Docker containers page to lean more how use Docker containers with Nextflow.

Scope singularity

The singularity configuration scope controls how Singularity containers are executedby Nextflow.

The following settings are available:

Name

Description

enabled

Turn this flag to true to enable Singularity execution (default: false).

engineOptions

This attribute can be used to provide any option supported by the Singularity engine i.e. singularity[OPTIONS].

envWhitelist

Comma separated list of environment variable names to be included in the container environment.

runOptions

This attribute can be used to provide any extra command line options supported by the singularityexec.

noHttps

Turn this flag to true to pull the Singularity image with http protocol (default: false).

autoMounts

When true Nextflow automatically mounts host paths in the executed container. It requires the user bind control feature enabled in your Singularity installation (default: false).

cacheDir

The directory where remote Singularity images are stored. When using a computing cluster it must be a shared folder accessible to all computing nodes.

pullTimeout

The amount of time the Singularity pull can last, exceeding which the process is terminated (default: 20min).

Read Singularity containers page to lean more how use Singularity containers with Nextflow.

Scope podman

The podman configuration scope controls how Podman containers are executed by Nextflow.

The following settings are available:

Name

Description

enabled

Turn this flag to true to enable Podman execution (default: false).

envWhitelist

Comma separated list of environment variable names to be included in the container environment.

temp

Mounts a path of your choice as the /tmp directory in the container. Use the special value auto to create a temporary directory each time a container is created.

remove

Clean-up the container after the execution (default: true).

runOptions

This attribute can be used to provide any extra command line options supported by the podmanrun command.

registry

The registry from where container images are pulled. It should be only used to specify a private registry server. It should NOT include the protocol prefix i.e. http://.

engineOptions

This attribute can be used to provide any option supported by the Docker engine i.e. podman[OPTIONS].

mountFlags

Add the specified flags to the volume mounts e.g. mountFlags = ‘ro,Z’

The above options can be used by prefixing them with the podman scope or surrounding them by curlybrackets, as shown below:

Read Podman containers page to lean more how use Podman containers with Nextflow.

Scope manifest

The manifest configuration scope allows you to define some meta-data information needed when publishing your pipeline project on GitHub, BitBucket or GitLab, or when running your pipeline.

The following settings are available:

Name

Description

author

Project author name (use a comma to separate multiple names).

defaultBranch

Git repository default branch (default: master).

recurseSubmodules

Turn this flag to true to pull submodules recursively from the Git repository

description

Free text describing the workflow project.

doi

Project related publication DOI identifier.

homePage

Project home page URL.

mainScript

Project main script (default: main.nf).

name

Project short name.

nextflowVersion

Minimum required Nextflow version.

version

Project version number.

The above options can be used by prefixing them with the manifest scope or surrounding them by curlybrackets. For example:

To learn how to publish your pipeline on GitHub, BitBucket or GitLab code repositories read Pipeline sharingdocumentation page.

Nextflow version¶

The nextflowVersion setting allows you to specify a minimum required version to run the pipeline.This may be useful to ensure that a specific version is used:

Scope trace

The trace scope allows you to control the layout of the execution trace file generated by Nextflow.

The following settings are available:

Name

Description

enabled

When true turns on the generation of the execution trace report file (default: false).

fields

Comma separated list of fields to be included in the report. The available fields are listed at this page

file

Trace file name (default: trace.txt).

sep

Character used to separate values in each row (default: t).

raw

When true turns on raw number report generation i.e. date and time are reported as milliseconds and memory as number of bytes

The above options can be used by prefixing them with the trace scope or surrounding them by curlybrackets. For example:

To learn more about the execution report that can be generated by Nextflow read Trace report documentation page.

Scope aws

The aws scope allows you to configure the access to Amazon S3 storage. Use the attributes accessKey and secretKeyto specify your bucket credentials. For example:

Click the following link to lean more about AWS Security Credentials.

Advanced client configuration options can be set by using the client attribute. The following properties can be used:

Name

Description

connectionTimeout

The amount of time to wait (in milliseconds) when initially establishing a connection before giving up and timing out.

endpoint

The AWS S3 API entry point e.g. s3-us-west-1.amazonaws.com.

maxConnections

The maximum number of allowed open HTTP connections.

maxErrorRetry

The maximum number of retry attempts for failed retryable requests.

protocol

The protocol (i.e. HTTP or HTTPS) to use when connecting to AWS.

proxyHost

The proxy host to connect through.

proxyPort

The port on the proxy host to connect through.

proxyUsername

The user name to use when connecting through a proxy.

proxyPassword

The password to use when connecting through a proxy.

signerOverride

The name of the signature algorithm to use for signing requests made by the client.

socketSendBufferSizeHint

The Size hint (in bytes) for the low level TCP send buffer.

socketRecvBufferSizeHint

The Size hint (in bytes) for the low level TCP receive buffer.

socketTimeout

The amount of time to wait (in milliseconds) for data to be transferred over an established, open connection before the connection is timed out.

storageEncryption

The S3 server side encryption to be used when saving objects on S3 (currently only AES256 is supported)

userAgent

The HTTP user agent header passed with all HTTP requests.

uploadMaxThreads

The maximum number of threads used for multipart upload.

uploadChunkSize

The size of a single part in a multipart upload (default: 10 MB).

uploadStorageClass

The S3 storage class applied to stored objects, one of [STANDARD, STANDARD_IA, ONEZONE_IA, INTELLIGENT_TIERING] (default: STANDARD).

uploadMaxAttempts

The maximum number of upload attempts after which a multipart upload returns an error (default: 5).

uploadRetrySleep

The time to wait after a failed upload attempt to retry the part upload (default: 100ms).

For example:

Advanced Batch configuration options can be set by using the batch attribute. The following properties can be used (required version 19.07.0 or later):

Name

Description

cliPath

The path where the AWS command line tool is installed in the host AMI.

jobRole

The AWS Job Role ARN that needs to be used to execute the Batch Job.

volumes

One or more container mounts. Mounts can be specified as simple e.g. /some/path or canonical format e.g. /host/path:/mount/path[:ro|rw]. Multiple mounts can be specifid separating them with a comma or using a list object.

delayBetweenAttempts

Delay between download attempts from S3 (default 10 sec).

maxParallelTransfers

Max parallel upload/download transfer operations per job (default: 16).

maxTransferAttempts

Max number of downloads attempts from S3 (default: 1).

Scope cloud

Note

The cloud configuration scope has been retired.

Scope conda

The conda scope allows for the definition of the configuration settings that control the creation of a Conda environmentby the Conda package manager.

The following settings are available:

Name

Description

cacheDir

Defines the path where Conda environments are stored. When using a compute cluster make sure to provide a shared file system path accessible from all computing nodes.

createTimeout

Defines the amount of time the Conda environment creation can last. The creation process is terminated when the timeout is exceeded (default: 20min).

Scope k8s

The k8s scope allows the definition of the configuration settings that control the deployment and execution ofworkflow applications in a Kubernetes cluster.

The following settings are available:

Name

Description

autoMountHostPaths

Automatically mounts host paths in the job pods. Only for development purpose when using a single node cluster (default: false).

context

Defines the Kubernetes configuration context name to use.

namespace

Defines the Kubernetes namespace to use (default: default).

serviceAccount

Defines the Kubernetes service account name to use.

launchDir

Defines the path where the workflow is launched and the user data is stored. This must be a path in a shared K8s persistent volume (default: <volume-claim-mount-path>/<user-name>.

workDir

Defines the path where the workflow temporary data is stored. This must be a path in a shared K8s persistent volume (default:<user-dir>/work).

projectDir

Defines the path where Nextflow projects are downloaded. This must be a path in a shared K8s persistent volume (default: <volume-claim-mount-path>/projects).

pod

Allows the definition of one or more pod configuration options such as environment variables, config maps, secrets, etc. It allows the same settings as the pod process directive.

pullPolicy

Defines the strategy to be used to pull the container image e.g. pullPolicy:'Always'.

runAsUser

Defines the user ID to be used to run the containers.

storageClaimName

The name of the persistent volume claim where store workflow result data.

storageMountPath

The path location used to mount the persistent volume claim (default: /workspace).

storageSubPath

The path in the persistent volume to be mounted (default: root).

volumeClaims

(deprecated)

See the Kubernetes documentation for more details.

Scope timeline

The timeline scope allows you to enable/disable the processes execution timeline report generated by Nextflow.

The following settings are available:

Name

Description

enabled

When true turns on the generation of the timeline report file (default: false).

file

Timeline file name (default: timeline.html).

Scope mail

The mail scope allows you to define the mail server configuration settings needed to send email messages.

Name

Description

from

Default email sender address.

smtp.host

Host name of the mail server.

smtp.port

Port number of the mail server.

smtp.user

User name to connect to the mail server.

smtp.password

User password to connect to the mail server.

smtp.proxy.host

Host name of an HTTP web proxy server that will be used for connections to the mail server.

smtp.proxy.port

Port number for the HTTP web proxy server.

smtp.*

Any SMTP configuration property supported by the Java Mail API (see link below).

debug

When true enables Java Mail logging for debugging purpose.

Note

Nextflow relies on the Java Mail API to send email messages.Advanced mail configuration can be provided by using any SMTP configuration property supported by the Java Mail API.See the table of available properties at this link.

For example, the following snippet shows how to configure Nextflow to send emails through theAWS Simple Email Service:

Scope notification

The notification scope allows you to define the automatic sending of a notification email messagewhen the workflow execution terminates.

Name

Description

enabled

Enables the sending of a notification message when the workflow execution completes.

to

Recipient address for the notification email. Multiple addresses can be specified separating them with a comma.

from

Sender address for the notification email message.

template

Path of a template file which provides the content of the notification message.

binding

An associative array modelling the variables in the template file.

The notification message is sent my using the STMP server defined in the configuration mail scope.

If no mail configuration is provided, it tries to send the notification message by using the external mail commandeventually provided by the underlying system (eg. sendmail or mail).

Scope report

The report scope allows you to define configuration setting of the workflow Execution report.

Name

Description

enabled

If true it create the workflow execution report.

file

The path of the created execution report file (default: report.html).

Scope weblog

The weblog scope allows to send detailed trace scope information as HTTP POST request to a webserver, shipped as a JSON object.

Detailed information about the JSON fields can be found in the weblog description.

Name

Description

enabled

If true it will send HTTP POST requests to a given url.

url

The url where to send HTTP POST requests (default: http:localhost).

Config profiles¶

Configuration files can contain the definition of one or more profiles. A profile is a set of configuration attributesthat can be activated/chosen when launching a pipeline execution by using the -profile command line option.

Configuration profiles are defined by using the special scope profiles which group the attributes that belongto the same profile using a common prefix. For example:

This configuration defines three different profiles: standard, cluster and cloud that set different processconfiguration strategies depending on the target runtime platform. By convention the standard profile is implicitly usedwhen no other profile is specified by the user.

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Tip

Two or more configuration profiles can be specified by separating the profile nameswith a comma character, for example:

Danger

When using the profiles feature in your config file do NOT set attributes in the same scope bothinside and outside a profiles context. For example:

In the above example the process.cpus attribute is not correctly applied because the process scope is alsoused in the foo and bar profile contexts.

The above feature requires version 0.28.x or higher.

Environment variables¶

The following environment variables control the configuration of the Nextflow runtime andthe Java virtual machine used by it.

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Name

Description

NXF_HOME

Nextflow home directory (default: $HOME/.nextflow).

NXF_VER

Defines what version of Nextflow to use.

NXF_ORG

Default organization prefix when looking for a hosted repository (default: nextflow-io).

NXF_GRAB

Provides extra runtime dependencies downloaded from a Maven repository service.

NXF_OPTS

Provides extra options for the Java and Nextflow runtime. It must be a blank separated list of -Dkey[=value] properties.

NXF_CLASSPATH

Allows the extension of the Java runtime classpath with extra JAR files or class folders.

NXF_ASSETS

Defines the directory where downloaded pipeline repositories are stored (default: $NXF_HOME/assets)

NXF_PID_FILE

Name of the file where the process PID is saved when Nextflow is launched in background.

NXF_WORK

Directory where working files are stored (usually your scratch directory)

NXF_TEMP

Directory where temporary files are stored

NXF_DEBUG

Defines scripts debugging level: 1 dump task environment variables in the task log file; 2 enables command script execution tracing; 3 enables command wrapper execution tracing.

NXF_EXECUTOR

Defines the default process executor e.g. sge

NXF_CONDA_CACHEDIR

Directory where Conda environments are store. When using a computing cluster it must be a shared folder accessible from all computing nodes.

NXF_SINGULARITY_CACHEDIR

Directory where remote Singularity images are stored. When using a computing cluster it must be a shared folder accessible from all computing nodes.

NXF_JAVA_HOME

Defines the path location of the Java VM installation used to run Nextflow. This variable overrides the JAVA_HOME variable if defined.

NXF_OFFLINE

When true disables the project automatic download and update from remote repositories (default: false).

NXF_CLOUD_DRIVER

Defines the default cloud driver to be used if not specified in the config file or as command line option, either aws or google.

NXF_ANSI_LOG

Enables/disables ANSI console output (default true when ANSI terminal is detected).

NXF_ANSI_SUMMARY

Enables/disables ANSI completion summary: true|false (default: print summary if execution last more than 1 minute).

NXF_SCM_FILE

Defines the path location of the SCM config file (requires version 20.10.0 or later).

NXF_PARAMS_FILE

Defines the path location of the pipeline parameters file (requires version 20.10.0 or later).

JAVA_HOME

Defines the path location of the Java VM installation used to run Nextflow.

JAVA_CMD

Defines the path location of the Java binary command used to launch Nextflow.

HTTP_PROXY

Defines the HTTP proxy server

HTTPS_PROXY

Defines the HTTPS proxy server